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Fig. 1 | Cell & Bioscience

Fig. 1

From: Rapid and robust derivation of mesenchymal stem cells from human pluripotent stem cells via temporal induction of neuralized ectoderm

Fig. 1

Generation and characterization of human iPSCs with an integration-free minicircle vector. a Morphology of human primary dermal fibroblasts (1), iPSCs generated from fibroblasts cultured on feeder layer (2) and feeder-free plate (3). Scar bar 100 μm. b Expression of pluripotency-related genes OCT3/4, NANOG, SOX2, REX1, GDF3, hTERT and DNMT3b, analyzed by qPCR. Gene expression was normalized to endogenous GAPDH and was plotted against fibroblast, H9 served as a positive control. Data represent mean ± S.D. n = 3. c DNA methylation detected by Bisulfite sequencing in the genomic region of OCT3/4 promoter in iPSCs and its parental dermal fibroblasts. Open and filled circles represents unmethylated and methylated CpGs, respectively. Each row represents bisulfite sequencing result from a given amplicon and ten amplicons were analyzed. d Immunostaining of pluripotency markers SSEA-4, TRA60-1, NANOG and OCT3/4 (Scar bar 10 μm), Alkaline Phosphatase (AP) staining and embryoid body (EB) formation of iPSCs. Scar bar 100 μm. e Karyotyping assay of iPSC with a normal diploid female karyotype. f Teratoma formation of iPSCs in the testis of NOD/SCID mice. Teratoma and H&E staining of paraffin sections to reveal three germ layers. Scar bar 100 μm

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