Fig. 5

Nuclear translocation of NICD upon starvation. A HeLa cells were starved in HBSS for 4 h with or without 20 μM chloroquine (CQ). Cytosolic and nuclear extracts were subjected to Western blotting. \(\alpha \)-tubulin and lamin A/C were used as loading controls for the cytosolic and nuclear fractions, respectively. B, C Quantification of NICD and SNAI1. Relative levels of NICD (B) and SNAI1 (C) in the cytosol and nucleus were quantified using NIH ImageJ software. Data represent the mean (± S.D.) of three independent experiments (**P < 0.01). D, E Localization of NICD. HeLa cells starved in HBSS in the presence of 20 μM chloroquine (CQ) were immunostained with anti-NICD antibodies. Images were captured under a florescent microscope (D), and quantified using NIH ImageJ software (E). Scale bar, 10 μm. Data represent the mean (± S.D.) of three independent experiments (**P < 0.01)