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Fig. 4 | Cell & Bioscience

Fig. 4

From: Role of ammonia for brain abnormal protein glycosylation during the development of hepatitis B virus-related liver diseases

Fig. 4

Ammonia stimulated C1GALTI expression and C1GALT1 silencing in astrocytes. a CIGALT1 and C1GALT1C1 mRNA levels in NH4Cl treated SVG p12, SW1088 and CCF-STTG1 cells were assessed by real-time PCR. b CIGALT1 mRNA levels in NH4Cl plus MSO or apocynin treated SVG p12, SW1088 and CCF-STTG1 cells were assessed by real-time PCR. c Western blotting of C1GALT1 protein levels in NH4Cl plus MSO or apocynin treated astrocytes compared with untreated astrocytes. d Ca2+ was loaded with Fluo-4 AM in NH4Cl treated and untreated SVG p12 and SW1088 cells and their fluorescence intensity of Ca2+. Fluorescent images were captured using the same condition, Scale Bar 80 μm. e Real-time PCR and western blotting of IP3R1 and mGluR5 levels in NH4Cl treated and untreated SVG p12 and SW1088 cells. f C1GALT1 levels in SVG p12 and SW1088 cells expressing siRNA targeting C1GALT1 (siC1GALT1-1 or siC1GALT1-2) or non-targeting control (siNTC) were determined by real-time PCR and western blotting. g IP3R1 levels of SVG p12 and SW1088 cells transduced with siC1GALT1-1, siC1GALT1-2 or siNTC were assessed by real-time PCR and western blotting. Real-time PCR graph shows average relative expression normalized to GAPDH, and data are from at three independent cultures performed in least three times

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