Fig. 3

Ammonia-induced alternations of protein glycosylation in astrocytes. a The glycopatterns of Cy3-labeled protein of SVG p12, SW1088 and CCF-STTG1 cells bound to the lectin microarrays, and the NFIs of 10 lectins in the 0.5, 2, 5 or 10 mmol/L NH₄Cl treated and untreated cells. b The fluorescence-based lectin cytochemical and average fluorescence intensity of Galβ1-3GalNAc binder MPL in 5 mmol/L NH₄Cl treated compared with untreated SVG p12, SW1088 and CCF-STTG1 cells. c The glycopatterns of Cy3-labeled protein of SVG p12, SW1088 and CCF-STTG1 cells bound to the lectin microarrays and their NFIs, cells were treated with NH₄Cl plus the glutamine synthetase inhibitor (L-Methionine sulfoximine, MSO), NADPH oxidase inhibitor (apocynin) or CH₃NH₃Cl. MPL was marked in the white boxes. d The fluorescence-based lectin cytochemical and average fluorescence intensity of Galβ1-3GalNAc binder MPL in SVG p12, SW1088 and CCF-STTG1 cells, which treated with NH₄Cl plus the glutamine synthetase inhibitor (L-Methionine sulfoximine, MSO) or NADPH oxidase inhibitor (apocynin). The images were acquired using the same condition and shown on the same scale in the Cy5- and DAPI-merge channel, Scale Bar 80 μm