Fig. 6

In vivo TF administration during HFD upregulates ILK in WAT and modifies the genetic pattern of several markers in WAT depots. Control mice (WT) were challenged to a high-fat diet (HFD) or standard diet (STD) for 2 weeks and subjected to TF (50 microg/Kg/day, i.p.) or vehicle (VH). Afterward, animals were fasted overnight, weighed, sacrificed and WAT depots (WAT) were dissected. A Fold changes of ILK mRNA expression analyzed by RT-qPCR and normalized to β-actin in epiWAT from STD and HFD WT. B Representative immunoblots and densitometric analysis of AKT phosphorylated at Ser473 (P-AKT) and GSK3β phosphorylated at Ser9 (P-GSK), normalized to total protein content (transference membrane dyed with ponceau) in epiWAT of HFD WT. C HFD WT epiWAT mRNA expression fold changes, analyzed by RT-qPCR and normalized to β-actin, of markers for inflammation (IL6 and MCP1), adipogenesis, (PPARγ, Leptin), proliferation (PCNA), browning (UCP-1). D HFD WT scWAT mRNA expression fold changes of ILK and UCP1. E HFD WT epiWAT mRNA expression fold changes of lipid metabolism markers FABP4, FAT, AQP7, FASN, HSL, ATGL. N = 6–12. *p < 0.05 vs WT STD VH, #p < 0.05 vs WT HFD VH