Fig. 4

TF binding to INTB1 increases ILK expression and activity and modifies the expression of other markers to reduce TG content. Deprived differentiated adipocytes from C3H10T1/2 were transfected with specific siRNAs against ILK (siILK) or scrambled siRNAs as transfection control. 48 h after transfection, cells were deprived and treated with TF 50 µM or vehicle (CT) or co-treated with specific INTB1 blocking antibody (HMB1) at the indicated times. A Effect of 24 h TF and siILK transfection on ILK mRNA expressions fold change, analyzed by RT-qPCR and normalized to β-actin, and B TG contain stained with AdipoRed. C Representative immunoblots and densitometric analysis of AKT phosphorylated at ser 473 (P-AKT) and D GSK3β phosphorylated at ser 9 (P-GSK), normalized to the content of total AKT or GSK3β proteins respectively from control cells treated with TF 4, 16, 24 h or vehicle 24 h. E effect of 24 h TF and HMB1 on ILK mRNA expressions fold change, analyzed by RT-qPCR and normalized to β-actin and F effect of 24 h TF and HMB1 on P-AKT; representative immunoblots and densitometric analysis normalized to total AKT. G Effects of TF, siILK and HMB1 on adipogenesis marker PPARγ or H browning marker UCP1, analyzed by RT-qPCR and normalized to β-actin. N = 8–12. Data are shown as mean ± SEM, *p < 0.05 vs CT. #p < 0.05 vs TF