Fig. 3

TF reduces the levels of F-actin in adipocytes. Deprived differentiated adipocytes from c3H10T1/2 were treated with TF 50 µM or vehicle (CT) or co-treated with specific INTB1 blocking antibody (HMB1) for the indicated times. Representative confocal microscopy images of F-actin dyed with phalloidin (red) and nuclei with DAPI (blue) and quantification of F-actin/nuclei per image after A 60 min and B 24 h of treatment. Scale bars 50 μm. N = 8–12. Data are shown as mean ± SEM, *p < 0.05 vs CT, #p < 0.05 vs TF