Fig. 3
From: Transcriptome-scale spatial gene expression in rat arcuate nucleus during puberty
Cell clusters identification in ARC. A The tSNE plot showing the cells are classified into 4 clusters based on the transcriptomes of overall gene expression in 514 spots of ARC (Cluster 9) (Top). Feature plots showing the distribution of clusters in PND-25, 35 and 45 (Bottom). B Heatmap showing the top ten genes in these 4 subclusters. Red frame highlights the top expressed genes in Subcluster 1. C–G The IF assay showing the expression of Kiss1, Nr5a2, Spp1, Tgfbi and Slc18a3 in ARC of PND-25, 35 and 45 rat