Fig. 5

AIF blocks hypoxia-induced pulmonary vascular remodeling in vivo. A Morphological analysis of the pulmonary artery was performed using HE staining and Masson staining, and the thickness of pulmonary vascular medium was measured by α-SMA staining (n = 5). B Increased proliferation of the pulmonary vascular cells was visualized by PCNA-positive staining per vascular area under hypoxia compared with exposure to normal conditions at the same time, these effects were reversed by the administration of AAV5-AIF (n = 3). C Homology analysis of the AIF gene among humans, mice and rats. Nor normoxia, Hyp hypoxia, NC negative control