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Fig. 3 | Cell & Bioscience

Fig. 3

From: YBX1 mediates alternative splicing and maternal mRNA decay during pre-implantation development

Fig. 3

Knockdown of YBX1 Compromised the transcriptional activity during ZGA. A Unsupervised clustering of genes in YBX1 knockdown and the control embryos. B Volcano plot of gene expression after YBX1 knockdown. C Heatmap of DEGs at the 8-cell stage YBX1 knockdown embryos and the controls. D Two clusters of the DEGs. E Representative images 5-ethynyl uridine (5-EU, red) and DAPI staining (blue) at the 4- and 8-cell embryos, and the 8-cell stage YBX1 knockdown embryos in goat. Scale bar = 20 μm. F Statistical analysis of 5-EU signal intensity in 4- and 8-cell embryos, and the 8-cell stage YBX1 knockdown embryos in goat. Values are presented as mean ± SEM and compared using student’s t-test. G Representative images 5-EU (red) and DAPI staining (blue) at the 2-cell stage YBX1 knockdown embryos and the controls in mice. Scale bar = 10 μm. H Statistical analysis of 5-EU signal intensity in 2-cell stage YBX1 knockdown embryos and the controls in mice. Values are presented as mean ± SEM and compared using student’s t-test

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