Fig. 9

Host Rpb3, Rpb6, and Rpb11 were involved in ADRV infection in BHK-21 cells. A–C Localization of Rpb3 (A), Rpb6 (B), and Rpb11 (C) in viral factories in ADRV infected BHK-21 cells. The cells were infected with or without ADRV at an MOI of 1 and fixed at the indicated time points. Rpb3, Rpb6, and Rpb11 (red) were stained with commercial antibodies. Replicating DNA was stained with EdU (green) as described above. Cell nuclei were stained with DAPI (blue). Bar = 5 μM. D–K Inhibition of the expression of Rpb3, Rpb6, and Rpb11 reduced ADRV gene expression in BHK-21 cells. siRNAs targeting Rpb3, Rpb6, and Rpb11 were transfected into the cells. The transfected cells were subjected to western blotting analysis (D–F) or ADRV infection. Expression of the ICP18 gene (G–I) and MCP gene (J, K) at different time points was determined by RT–qPCR