Fig. 7
Identification of ADRV transcription machinery related proteins with an ADRV46R-3Flag recombinant virus. A Construction of recombinant ADRV46R-3Flag expressing the 46R-3Flag fusion protein. A 3× Flag tag was fused to the C-terminus of 46R. EGFP driven by the viral P50 promoter was used as a selection marker. The dashed line indicates the virus genome. B Fluorescence micrograph of recombinant virus infected cells showing the expression of EGFP. C Western blotting analysis of recombinant virus infected cells with anti-Flag antibody. 46R-3Flag expressed successfully. D Viral and host proteins identified by MS analysis