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Fig. 1 | Cell & Bioscience

Fig. 1

From: Replication and transcription machinery for ranaviruses: components, correlation, and functional architecture

Fig. 1

Determination of the conditions for iPOND in ADRV- and RGV-infected GSTC cells. A Visualization of DNA labeled by EdU in ADRV infections at 6 and 12 hpi. GSTC cells were infected with or without ADRV at 1 MOI. The cells were incubated for 1 h with 10 μM EdU at the indicated time points and then fixed, permeabilized, and reacted with Alexa Fluor 488 azide. Cellular DNA was stained with Hoechst 33342 (blue). The EdU-labeled DNA presented is shown in green color. Visible Hoechst-labeled cytoplasmic viral factories are indicated with arrows. B Kinetics of DNA synthesis of the two viruses. GSTC cells were exposed to ADRV and RGV for 1 h at 0.1 MOI, respectively, and collected at the indicated time points. Total DNA was extracted. Viral genome amounts were determined by qPCR detection of MCP copy numbers. The genome amounts after 1 h of adsorption were set as 1

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