Fig. 5
From: BVES is a novel interactor of ANO5 and regulates myoblast differentiation
Impact of BVES or ANO5 deficiency on myoblast proliferation and differentiation. A, B Quantitative RT-PCR analysis of Ano5 (A) and BVES (B) expression during C2C12 myoblast differentiation. **p < 0.01, ***p < 0.001, ****p < 0.0001 (one-way ANOVA with Turkey’s post tests). C The CCK8 proliferation assay of WT, BVES-KO and Ano5-KO C2C12 myoblasts from 12 to 84 h in culture. D Immunofluorescence images of WT, BVES-KO and Ano5-KO C2C12 myotubes on day 4 of differentiation stained with the monoclonal antibody against myosin heavy chain (MHC) and DAPI. Scale bar: 100 µm. E Quantification of the fusion index of WT, BVES-KO and Ano5-KO C2C12 myotubes in differentiation medium for four days. **p < 0.01 (one-way ANOVA with Turkey’s post tests)