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Fig. 2 | Cell & Bioscience

Fig. 2

From: BVES is a novel interactor of ANO5 and regulates myoblast differentiation

Fig. 2

Validation of BVES-ANO5 interaction by Co-IP. A ANO5 was co-immunoprecipitated with BVES-myc and POPDC2-myc using the myc antibody from the lysates of Cos-1 cells with co-expression of ANO5 and BVES or POPDC2. B BVES was co-immunoprecipitated with FLAG-hANO5-BioID2 using the FLAG antibody from the lysates of C2C12 with co-expression of FLAG-hANO5-BioID2 and BVES-myc. C Schematic view of the mutant ANO5 and BVES constructs. D BVES-myc efficiently pulled down the N-terminal of Ano5. E Mapping the N-terminal ANO5 fragments interacting with BVES. F Mapping the BVES domains interacting with ANO5

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