Fig. 4

Effects of MCTR1 on LPS-induced lipid peroxidation and ferroptosis in vitro. A Viability curves for HK-2 cells treated with different concentrations (0, 1, 3, 10, 30, 100, and 300 nM) of MCTR1 and LPS (1 ug/ml) for 8 h. B Viability curves for HK-2 cells treated with different concentrations (0, 1, 3, 10, 30, 100, and 300 nM) of MCTR1 for 0 and 8 h. C HK-2 cells treated with MCTR1 (100 nM) and LPS (1 ug/ml) for 8 h, and the visualization of cell viability were evaluated by phase-contrast microscopy. D, E Representative western blotting and quantification of GPX4 and PTGS2 protein. F–H Quantitative analyses of MDA, GSH and, non-heme iron. I Quantitative analyses of oxidized C11-BODIPY 581/591 probe by flow cytometry. J Representative images of C11-BODIPY 581/591 fluorescent ratio-probe. K Representative TEM images. The black arrow indicates ferroptosis-like mitochondria. L Quantitative analyses of mitochondrial length. n = 3, mean ± SD were presented. **P < 0.01