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Fig. 7 | Cell & Bioscience

Fig. 7

From: From nasal to basal: single-cell sequencing of the bursa of Fabricius highlights the IBDV infection mechanism in chickens

Fig. 7

The expression of chicken interferon pathways after IBDV infection. a Pie chart showing the percentage of gene transcriptome level in designated cell type, graph showing the gene transcriptome level in control and infected groups in each cell type, and t-SNE distribution of gene transcriptome level in throughout the bursal cells population; of IFN-β; b IFNAR1 & IFNAR2 (type 1 IFN surface receptors); c IFN-γ and; d IFNGR1 & IFNGR2 (IFN-γ surface rectors). e Morphological observation of DF1 cells stimulated by Poly I:C and IBDV at 12hpi and 24hpi through the light microscope. f Plaque appearance of DF1 cells infected with the IBDV. Each group is shown in two different wells. Blank: mock cells; 10–1 to 10–5 show plaque formation by a tenfold dilution of virus stock. g Viral RNA expression level of IBDV segments (IBDV-A and IBDV-B) on DF1 cells followed by IBDV infection at the indicated time. h Relative mRNA expression level at DF1 cells after treatment with poly I:C and IBDV at 12hpi and 24hpi of IFN-α & IFN-β, and protein expression through ELISA of IFN-α (right side). i Relative mRNA expression level at DF1 cells after treatment with poly I:C and IBDV at 12hpi and 24hpi of IFN-γ & IFNGR1 and protein expression through ELISA of IFN-γ (right side).j IBDV-A and IBDV-B mRNA expression level through qRT-PCR after post-treatment on DF1 cells with synthetic IFN-γ followed by IBDV infection for the indicated time. All the experiments are the means ± standard error from three independent repeats. All the qRT-PCR expressions were normalized with GAPDH mRNA expression level. The level of significance between blank and treated groups are identified by *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001, determined by one-way ANOVA with Tukey’s multiple comparison test

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