Fig. 6

Epithelial cells distribution in bursal cell’s population and IBDV distribution in the subtype of epithelial cells. a t-SNE representation of the major five cell types shown in different color distribution. The encircled yellow cells showed the aggregate epithelial cells population. b t-SNE visualization of the entire epithelial cells population classified into nine sub-populations based on the unique mRNA transcriptional profiling, shown in different colors. c Heatmap of the highly expressed genes in epithelial cells population shown in nine sub-population differences. d t-SNE visualization of epithelial cells into four subtypes (Basal cells in blue, Ionocytes in orange, goblet cells in green, and AT1/AT2 like cells in violet color) based on differential gene expression levels shown in a different color. e t-SNE representation of four epithelial cells subtypes into normal and IBDV infected hosts samples defined through color distribution. f t-SNE graph of the IBDV strain BC6/85-A and BC6/85-B viral load (shown in colored plus) in IBDV infected hosts samples. g tSNE graph of the KRT5 (shown in colored) in epithelial cells. h FACS analyses of KRT5 basal cells in Control and IBDV infected chicken bursa after 72 hpi. Bursal cells were isolated from each group and gated for KRT5 (marker of basal cells)