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Fig. 2 | Cell & Bioscience

Fig. 2

From: From nasal to basal: single-cell sequencing of the bursa of Fabricius highlights the IBDV infection mechanism in chickens

Fig. 2

Replication and distribution of IBDV on chickens’ blood and PBMCs. a RNA expression levels of control and IBDV infected chicken in blood and PBMC at different time points after intranasal IBDV inoculation, n = 3 from 3 chickens per group. b FACS analyses of control and intranasally IBDV challenged chickens at indicated times. PBMC were isolated from each group and gated for Bu1 (marker of B cells), CD4 + T cells, and c and d immunoglobulins (IgM + and IgY +), quantification of FACS IgM + and IgY + cells are shown in the histogram at indicated time. n = 4 from 3 chickens per group. All data shown are the mean results ± SD from three independent experiments. Statistical significance was determined using one-way ANOVA. Significance difference were ***p < 0.001, ****p < 0.0001

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