Skip to main content
Fig. 9 | Cell & Bioscience

Fig. 9

From: SIRT6 inhibition delays peripheral nerve recovery by suppressing migration, phagocytosis and M2-polarization of macrophages

Fig. 9

SIRT6 inhibition suppresses macrophages polarization toward M2 type. A, B Cell morphology of RAW264.7 and BMDM after M2 polarization with OSS_128167 treatment. The outlines of RAW264.7 and BMDM were stained by macrophages marker F4/80. Scale bar: 50 µm. C, D Western blots of CD163, Arg-1 expression after M2 polarization and OSS_128167 treatment in RAW264.7 and BMDM. E, F Quantification of CD163 and Arg-1 protein levels in RAW264.7 and BMDM. The protein levels were normalized with β-actin. *P  < 0.05. G Immunofluorescence staining for M2 macrophages marker CD163 in RAW264.7 and BMDM. Scale bar: 50 µm. H, I Quantifications of mean fluorescence intensity (au) of CD163 and ratio of CD163 positive cells (%) in RAW264.7 and BMDM. *P  < 0.05. OSS: OSS_128167, SIRT6 inhibitor

Back to article page