Fig. 8

SIRT6 inhibition promotes macrophages polarization toward M1 type. A, B Cell morphology of RAW264.7 and BMDM after M1 polarization and OSS_128167 treatment. The outlines of RAW264.7 and BMDM were stained by macrophages marker F4/80. Scale bar: 50 µm. C, D Western blots of iNOS, NF-κB, and TNF-α expression after M1 polarization and OSS_128167 treatment in RAW264.7 and BMDM. E, F Quantification of iNOS, NF-κB, and TNF-α protein levels in RAW264.7 and BMDM. The protein levels were normalized with β-actin. *P  < 0.05. G Immunofluorescence staining for iNOS in RAW264.7 and BMDM. Scale bar: 100 µm. H, I Quantifications of mean fluorescence intensity (au) of iNOS and ratio of iNOS positive cells (%) in RAW264.7 and BMDM. *P  < 0.05. OSS: OSS_128167, SIRT6 inhibitor