Fig. 6

SIRT6 expression is lower in M1 macrophages but higher in M2 macrophages. A Protocol of M1 and M2 polarization of macrophages. RAW264.7 and BMDM were induced to M1 type after stimulated with lipopolysaccharide (LPS) and IFN-γ, and induced to M2 type after stimulated with IL-4 and IL-13. B Phase contrast microscopy of RAW264.7 (left) BMDM (right) after polarization. M0 represents non-induced RAW264.7 or BMDM; M1 represents LPS and IFN-γ treated cells; M2 represents IL-4 and IL-13 treated cells. Scale bar: 20 µm. C, D Western blots of SIRT6, iNOS, CD163, and Arg-1 expression in the RAW264.7 and BMDM after polarization. E, F Quantification of SIRT6, iNOS, CD163, and Arg-1 expression in the RAW264.7 and BMDM after polarization. The protein levels were normalized with β-actin. *P  < 0.05. G, H qPCR analysis of Sirt6, iNOS, and Arg-1 mRNA levels in the RAW264.7 and BMDM after polarization. The mRNA level was normalized with GAPDH. *P  < 0.05