Fig. 4

SIRT6 expression is increased during macrophages phagocytosis and SIRT6 inhibition suppresses phagocytosis of macrophages. A Cell experiment protocol of macrophages phagocytosis. B Western blots of SIRT6, and MBP. C, D Quantification of SIRT6, and MBP protein levels in the blots. n.s: no significance; *P  < 0.05. E Protocol of BMDM treated with Vehicle or OSS_128167 (200 μM) for 24 h, and then 1 mg/ml myelin debris or 0.1 mg/ml fluorescence lumispheres (green) was added into the cultures for 4 h. F Double immunofluorescence staining for F4/80 stained macrophages and fluorescence lumispheres. Scale bar: 20 µm, 10 µm in the enlarged parts. G Quantification of the lumispheres number per macrophage. *P  < 0.05. H Double of immunofluorescence staining for phagocytosis of F4/80 stained macrophages and myelin debris (stained by ORO). Scale bar: 20 µm, 10 µm in the enlarged parts. I Quantification of lipid droplets per macrophage. *P  < 0.05. OSS_128167, SIRT6 inhibitor