Skip to main content
Fig. 3 | Cell & Bioscience

Fig. 3

From: SIRT6 inhibition delays peripheral nerve recovery by suppressing migration, phagocytosis and M2-polarization of macrophages

Fig. 3

SIRT6 inhibition suppresses macrophages migration. A Culture and identification of bone marrow-derived macrophages (BMDM). Left: phase contrast microscopy of BMDM; Right: immunofluorescence of macrophages maker F4/80 staining. Scale bar: 50 µm. B Transwell assay: the migrated macrophages in the Vehicle group and OSS_128167 treated group. Scale bar: 50 µm. C Quantification of migrated macrophages number, the number of migrated macrophages in OSS_128167 treated group is significantly decreased compared with that in the Vehicle group. *P  < 0.05. D Live/dead staining of BMDM treated with OSS_128167 (200 μM) after 24 h. The live cells were stained by Calcein-AM with green and the dead cells were stained by PI with red. Scale bar: 50 µm, 10 µm in the enlarged parts. E Quantification of live cells ratio, the ratio of live cells in the OSS_128167 group were similar to the Vehicle group. n.s: no significance; *P  < 0.05. F Scratch assay: after 4 h and 8 h, more slower gap closure (or healing area) was seen with BMDM of treated with OSS_128167. G Quantification of the relative healing area of scratch. *P  < 0.05. OSS_128167, SIRT6 inhibitor

Back to article page