Fig. 2

SIRT6 silencing in crushed sciatic nerve delays nerve and functional recovery. A Flow path of shControl or shSIRT6 lentivirus microinjection in crushed sciatic nerve of mice, the mice were received electrophysiology examination and tissue collection for Western blotting and histochemistry analysis after 3 weeks. B Gross image of shControl/shSIRT6 microinjection after nerve crush immediately. The aquamarine blue indicates fast green. C An intense GFP (green) labeling in the lentivirus injected sciatic nerve. Scale bar: 100 µm. D Compared with shControl-injected nerve, shSIRT6-injected nerve reveals more redness and swelling, indicates a severer inflammation response. E Western blotting of SIRT6, MAG and MBP in the shControl/shSIRT6 injected sciatic nerves at 3 weeks post-surgery. F–H Quantification of SIRT6, MAG and MBP levels in the blots. The protein levels were normalized with β-actin. *P  < 0.05. I Gross image and H&E staining of gastrocnemius muscles. Scale bar: 50 µm. J, K Quantification of wet weight and myofiber area of gastrocnemius muscles. *P  < 0.05. L Electrophysiological examination of the compound action potential in the shControl/shSIRT6-injected sciatic nerves. M, N Quantification of latency (ms) and potential amplitude (mV). *P  < 0.05