Fig. 5

Gm15441 reduces endogenous Txnip protein levels in vitro. A, B Quantitative real-time PCR analysis (A) and western blot analysis (B) of mouse hepatocytes AML12 transduced with adenovirus expressing empty vector as control or Gm15441-L, for 24 h, MOI = 50. Quantitation of western blot is shown in the right panel. Error bars are SEM, n = 3, *P < 0.05; C, D quantitative real-time PCR analysis (C) and western blot analysis (D) of mouse hepatocytes AML12 transfected with siLacZ as control and Gm15441-targeted siRNA (siGm15441), for 48 h. Quantitation of western blot is shown in the right panel; Error bars are SEM, n = 2–3, *P < 0.05