Fig. 3

Expression patterns of Gm15441 and Txnip are similar upon metabolic signal stimulation in vitro. A Quantitative real-time PCR analysis of mouse primary hepatocytes treated with 100 nM insulin for 24 h or 200 nM glucagon for 6 h. B Quantitative real-time PCR analysis of mouse primary hepatocytes transduced with adenovirus expressing YFP, FOXO1, or HNF4α for 24 h, MOI = 50; C quantitative real-time PCR analysis of mouse primary hepatocytes treated with 0.1% DMSO (vehicle), 100 µM WY, 20 µM GW7647, or 10 µM GW4064 for 24 h; D Western blot analysis of mouse primary hepatocytes treated with 0.1% DMSO (vehicle), 100 µM WY, 20 µM GW7647, or 10 µM GW4064 for 24 h. Quantitation is shown in the right panel. Error bars are SEM, n = 3, *P < 0.05