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Fig. 5 | Cell & Bioscience

Fig. 5

From: Development of a simple and miniaturized sandwich-like fluorescence polarization assay for rapid screening of SARS-CoV-2 main protease inhibitors

Fig. 5

The primary screening of a natural product library. a A general view for the primary screening. The red dashed line in the figure indicated 50% inhibition, and the inhibitory activity of candidate compound was higher than this defined red line. b The chemical structure of dieckol, a natural phlorotannin component extracted from Ecklonia cava. c The inhibitory activity of dieckol in the FP screening assay. The inhibitory analysis of dieckol in the absence or presence of 1 mM DTT was performed as described for GC-376. The IC50 value of dieckol was analyzed using GraphPad Prism 5.0. All assays were performed in triplicate. d Analysis of interaction between dieckol and Mpro using the SPR assay. The binding kinetics of dieckol (0, 6.25, 12.5, 25 µM) to immobilized Mpro was plotted according to the change of RIU value

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