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Fig. 4 | Cell & Bioscience

Fig. 4

From: Development of a simple and miniaturized sandwich-like fluorescence polarization assay for rapid screening of SARS-CoV-2 main protease inhibitors

Fig. 4

The quality assessment of the FP screening assay. a The inhibitory activity of GC-376 in a FP screening assay. Briefly, 30 µL mixture of 0.4 µM Mpro and GC-376 at the indicated concentrations was preincubated for 35 min at RT, and then 30 µL dilution of 40 nM FP tracer was added to initiate the proteolytic reaction. After addition of avidin, the mP value was measured to calculate the IC50 value of GC-376. Three independent experiments were performed. b Assessment of Z factor of the FP screening assay. GC-376 was used as a positive control, and the measured mP value from negative and positive wells were shown, respectively. The Z factor of this FP screening assay was 0.85, and a high signal window was represented by a black straight line. The experiment was performed in triplicate

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