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Fig. 7 | Cell & Bioscience

Fig. 7

From: Low-level laser prevents doxorubicin-induced skeletal muscle atrophy by modulating AMPK/SIRT1/PCG-1α-mediated mitochondrial function, apoptosis and up-regulation of pro-inflammatory responses

Fig. 7

LLL exposure attenuates Dox-induced apoptosis. C2C12 cells were treated with 2 µM doxorubicin (Dox) for a total of 24 h, in the LLL-treated group, cells were exposed to LLL 8 J/m2 before Dox treatment. In LLL plus AMPK, SIRT1, and PGC-1α silencing groups, cells were transfected with AMPK or SIRT1 or PGC-1α siRNA for 36 h before LLL exposure and Dox treatment. The expression level of Bcl-2, Bax, caspase 3, and β-actin were investigated using Western blot assay (A). Protein expression levels were quantified and presented by a bar chart (B–D). The TUNEL assay was used for detecting apoptotic DNA fragmentation using flow cytometry. The data were presented as the mean ± SD of three biological replicates at three separate times. (* indicating p < 0.05 compared with the control group; # indicating p < 0.05 compared to Dox group; & indicating p < 0.05 compared to only LLL exposure group)

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Cell & Bioscience

ISSN: 2045-3701

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