Fig. 7

Binding of EDP to the ERC potentiates monocyte transendothelial migration through NEU1. PMA-pre-stimulated HUVEC monolayers in transwells were incubated, or not, with DANA (400 µM) for 1 h at 37 °C before stimulation, or not, by κE (50 µg/mL), V14 + κE (molar ratio 2:1) or V14 peptide alone for 1 h at 37 °C. Calcein AM-labeled THP-1 monocytes were added to the upper chamber and allowed to migrate through the HUVEC monolayer into the lower chamber for 2 h at 37 °C. The upper chamber was then removed and the fluorescence intensity in the lower compartment was measured. Results were expressed as mean ± SEM of 3–4 independent experiments, each run in triplicate, and normalized to the control condition (without, w/o). Statistical analysis was performed by Student’s t-test (***p < 0.001; ns: non-significant)