Fig. 6
Binding of EDP to the ERC increases ICAM-1 binding on monocytes. a THP-1 monocytes (1 × 106/mL) were stimulated, or not, with κE (50 µg/mL) for 1 h at 37 °C, and incubated, or not, with rhICAM-1-Fc/PE-labeled anti-Fc complex for 30 min at room temperature. Data shown are representative of 5 independent experiments. Black trace, control cells; green trace, κE-stimulated cells; blue trace, control cells incubated with rhICAM-1-Fc; red trace, κE-stimulated cells incubated with rhICAM-1-Fc. b The graph shows the median fluorescence expressed as mean ± SEM of 5 independent experiments and statistical analysis was performed by Student’s t-test (*p < 0.05; ns, non-significant)