Fig. 4From: Characterization of novel interactions with membrane NEU1 highlights new regulatory functions for the Elastin Receptor Complex in monocyte interaction with endothelial cellsNEU1 interacts with ICAM-1 in human endothelial cells. a Detection of ICAM-1 expression by Western blot in whole lysate of HUVEC pre-stimulated, or not, with PMA (100 nM, overnight). b Colocalization between NEU1 and ICAM-1 at the cell surface of PMA-pre-stimulated HUVEC incubated, or not, with κE (50 µg/mL, 1 h) by confocal microscopy. Areas of colocalization at the plasma membrane were analyzed by ImageJ software and are indicated in white on the right panels. Scale bar: 10 µm. c Left panel, ICAM-1 was immunoprecipitated with a mouse monoclonal anti-ICAM-1 antibody from whole lysates of PMA-pre-stimulated HUVEC and co-immunoprecipitation of NEU1 was monitored by Western blot. The image is representative of 3 independent experiments. Right panel, blot quantification by densitometry analysis. Results are expressed as mean ± SEM of 3 independent experiments and normalized to the basal condition (without κE, w/o). Statistical analysis was performed by Student’s t-test (ns, non-significant). d NEU1 was immunoprecipitated with a mouse monoclonal anti-NEU1 antibody from whole lysates of PMA-pre-stimulated HUVEC and co-immunoprecipitation of ICAM-1 was monitored by Western blot. The image is representative of 3 independent experiments. Right panel: blot quantification by densitometry analysis. Results are expressed as mean ± SEM of 3 independent experiments and normalized to the basal condition (w/o). Statistical analysis was performed by Student’s t-test (ns, non-significant)Back to article page