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Fig. 1 | Cell & Bioscience

Fig. 1

From: Characterization of novel interactions with membrane NEU1 highlights new regulatory functions for the Elastin Receptor Complex in monocyte interaction with endothelial cells

Fig. 1

NEU1 interacts with β2 integrin in human monocytes. a Colocalization between NEU1 and β2 integrin at the cell surface of THP-1 monocytes stimulated, or not, by κE (50 µg/mL, 1 h) by confocal microscopy. Areas of colocalization at the plasma membrane were analyzed by ImageJ software and are indicated in white on the right panels. Scale bar: 10 µm. b Left panel, β2 integrin was immunoprecipitated with a mouse monoclonal anti-β2 integrin from whole lysates of THP-1 cells and co-immunoprecipitation of NEU1 was monitored by Western blot. The image is representative of 6 independent experiments. Right panel, blot quantification by densitometry analysis. Results are expressed as mean ± SEM of 6 independent experiments and normalized to the basal condition (without κE, w/o). Statistical analysis was performed by Student’s t-test (ns, non-significant). c NEU1 was immunoprecipitated with a mouse monoclonal anti-NEU1 antibody from whole lysates of THP-1 cells and co-immunoprecipitation of β2 integrin was monitored by Western blot. The image is representative of 6 independent experiments. Right panel: blot quantification by densitometry analysis. Results are expressed as mean ± SEM of 6 independent experiments and normalized to the basal condition (w/o). Statistical analysis was performed by Student’s t-test (ns, non-significant)

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