Fig. 3

Comparative evaluation of ASOs and siRNAs in testis. A The RNA level of Tsx detected by RT-PCR in control and knockdown testis treated with three different siRNAs. **p < 0.01, ***p < 0.001, n = 3. B RT-PCR analysis of Tsx expression in cytoplasm and nucleus 2 days after injection of si-Tsx-2 or ASO-Tsx-2. *p < 0.05, **p < 0.01, n = 3. C FISH of Tsx RNA (green) with IF of Vimentin (red). Testes were collected 2 days after injection of siRNAs or ASOs. Nuclei were stained with DAPI. Nuclei of Sertoli cells were indicated by white arrows. Scale bar, 20 μm. D RT-PCR results of Tsx RNA level 10 days after injection of si-Tsx-2 or ASO-Tsx-2. ****p < 0.0001, n = 3. E FISH images of Tsx RNA in testes 10 days after injection of si-Tsx-2 or ASO-Tsx-2. Nuclei were stained with DAPI. Scale bar, 50 μm. A, B, D Values are expressed as mean ± SD. Statistical significance was determined using t tests