Fig. 1
From: Concurrent mapping of multiple epigenetic marks and co-occupancy using ACT2-seq
Enrichment profiles generated using ACT2-seq are robust and reproducible. A Depiction of the ACT2 adapter design showing its three constitutive regions (top). Illustration of the concept of dual binding in ACT2 (bottom). Each antibody targets a distinct epigenetic factor and facilitates incorporation of a barcoded adapter via the associated Tn5 transposase subunit. B Genome browser snapshot of a representative genomic region enriched for “active” chromatin marks. ENCODE-validated ChIP-seq data sets are provided for comparison. IgG serves as the non-specific control for enrichment. C Genome browser snapshot of a representative genomic region enriched for H3K9me3. ENCODE-validated ChIP-seq data are provided for comparison. D Scatter plots examining peak enrichment correlation between two biological replicates of the same samples. Each data point represents the read density of a single enriched region