Fig. 6From: Catalase deficiency facilitates the shuttling of free fatty acid to brown adipose tissue through lipolysis mediated by ROS during sustained fastingUCP1 activation in brown adipocytes induced by ROS was suppressed by N-Acetylcysteine in catalase deficient adipocytes. A Representative fluorescence images primary brown adipocytes treated as indicated and fluorescence were measured with DCFH-DA and examined under a fluorescence microscope. Scale bar represents 100 μm. B Percentage of cells showing green fluorescence (corresponding to DCFH-DA) and fluorescence intensity of adipocytes. *P < 0.05 WT ISO vs KO ISO; **P < 0.001 KO ISO vs KO ISO + NAC C Proteins were extracted from treated adipocytes and immunoblot analysis was performed using whole cell lysates with the indicated antibodies. qPCR analysis of D UCP1: *P < 0.01 WT nor vs WT ISO; WT ISO vs KO ISO; KO ISO vs KO ISO + NAC. E PGC-1α: **P < 0.05 WT ISO vs KO ISO; KO ISO vs KO ISO + NAC and F PPARα in isolated brown adipocytes. Data represent mean ± SD of three independent experimentsBack to article page