Fig. 3

Catalase deficiency during sustained fasting increased BAT thermogenesis through mitochondrial activation. A Representative H&E staining of BAT from mice. Scale bar represents 20 μm. B Proteins were extracted from BAT of WT and KO mice and immunoblot analysis was performed using whole-cell lysates with the indicated antibodies (n = 3). α-Tubulin was used as a loading control. qPCR analysis of C UCP1 and D PGC-1α genes in BAT from mice. E Rectal body temperature was measured from mice fasted for indicated time (n = 6). F qPCR analysis of ADBR3. G, H Activities of mitochondrial complexes I and IV from BAT of mice. Details are described in Materials and Methods. I Total ROS was measured from BAT. Values represent mean ± SD (n = 3, 4). *P < 0.05 WT 48 h fasting versus KO 48 h of fasting