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Fig. 1 | Cell & Bioscience

Fig. 1

From: Catalase deficiency facilitates the shuttling of free fatty acid to brown adipose tissue through lipolysis mediated by ROS during sustained fasting

Fig. 1

Catalase deficiency decreases the accumulation of lipid in liver during sustained fasting. Liver samples from mice fasted at the indicated time were homogenized, and TG levels were analyzed from A liver and B serum (n = 6). C Liver tissues from mice cryosectioned and stained with ORO. Scale bar represents 20 μm. D Immunoblot analysis of liver tissue. Protein expression using whole-cell lysates with the indicated antibodies (n = 3). E qPCR analysis of FA metabolism-related genes from the liver of mice fed and fasted for 48 h. F β-hydroxybutyrate was analyzed from the serum of mice fasted for indicated time. G GC–MS for total FA of long Chain and VLCFA from liver samples (n = 3). Values represent mean ± SD (n = 3, 4). *P < 0.001 WT 48 h fasting versus KO 48 h of fasting

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Cell & Bioscience

ISSN: 2045-3701

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