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Fig. 9 | Cell & Bioscience

Fig. 9

From: NME6 is a phosphotransfer-inactive, monomeric NME/NDPK family member and functions in complexes at the interface of mitochondrial inner membrane and matrix

Fig. 9

Proximity ligation assays reveal association of NME proteins with OPA1 and RCC1L. a–c Proximity ligation assays (PLA) using MDA-MB-231T cells (Ctrl) or clones stably overexpressing NME6-186-FLAG (KI-186-18). Pictures show merged nuclear (green) and PLA stain (red), and PLA stain only (grey). PLA directed against a NME6/FLAG-NME6, NME6/OPA1 and NME6/RCC1L, b NME6/ANT, NME6/MFN and FLAG-NME6/DRP1, c NME4/FLAG-NME6, NME4/OPA1 and NME4/RCC1L. d Dual immunofluorescence stain of NME6 (green) and RCC1L (red) revealing colocalization in both Ctrl and KI-186-18 cells. PLA for NME6/FLAG-NME6 identifies two epitopes on the same protein as in KI-186-18 cells (positive control), but fails when FLAG-NME6 is lacking as in Ctrl cells (negative control). The NME4/FLAG PLA detects NME4/NME6 association, but is only applicable to KI-186-18 cells. (All scalebars: 10 µm)

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Cell & Bioscience

ISSN: 2045-3701

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