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Fig. 8 | Cell & Bioscience

Fig. 8

From: NME6 is a phosphotransfer-inactive, monomeric NME/NDPK family member and functions in complexes at the interface of mitochondrial inner membrane and matrix

Fig. 8

Stable overexpression of NME6 does not affect mitochondrial complex II, but downregulates complex III protein abundance. a Ten micrograms of cell lysate from MDA-MB-231T (Ctrl), silenced with scramble control (siCtrl), silenced for NME6 (siNME6) and stable clones overexpressing NME6-186-FLAG (KI-186-7, KI-186-18, KI-186-26) were analyzed by Western blot, using an antibody cocktail against OXPHOS complexes C-I to C-V (ab110412). Band intensity values were normalized to naphthol blue staining, and displayed as a ratio relative to b siCtrl or c Ctrl. The experiment was realized in triplicate (n = 3). Statistical analysis was performed using b Student’s test or c one-way ANOVA. All data are given as mean ± SD. Significances are given as ** p < 0.01; *** p < 0.001

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Cell & Bioscience

ISSN: 2045-3701

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