Fig. 4
Endogenous NME6 does not interact with Group I NME proteins. Co-immunoprecipitations were performed in a forward and reverse manner between Group I NME proteins and endogenous NME6 protein in MDA-MB-231T cells. Input proteins (I), supernatant depleted of immunoprecipitated proteins (Sn) and eluted proteins (IP) were analyzed by Western blot. a Endogenous NME proteins were immunoprecipitated using anti-NME1 or anti-NME6 coupled with Dynabeads™ protein G. Western blots were done using anti-NME1/2 antibody against NME1 (
) and NME2 (
), and anti-NME6. Proteins from cells transiently transfected to express b NME3-FLAG or c NME4-FLAG were pulled-down using FLAG-agarose or anti-NME6 coupled with Dynabeads™ protein G. Western blots were revealed using anti-FLAG and anti-NME6. NME4-FLAG displays two bands corresponding to NME4-full-lenght (
) and NME4-truncated after mitochondrial import (
). NME6 shows no interaction with Group I NME proteins