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Fig. 2 | Cell & Bioscience

Fig. 2

From: NME6 is a phosphotransfer-inactive, monomeric NME/NDPK family member and functions in complexes at the interface of mitochondrial inner membrane and matrix

Fig. 2

Endogenous NME6 is predominantly expressed as the 186 aa isoform in human cancer cell lines. a NME6 expression in a panel of human cancer and non-cancer cell lines. Twenty micrograms of total cell lysate was loaded on SDS-PAGE and analyzed by Western blot using anti-NME6 antibody. b Western blot analysis of MDA-MB-231T cell lysates (control) and MDA-MB-231T stable clones overexpressing NME6-194-FLAG (KI-194-2) and NME6-186-FLAG (KI-186-1) proteins, using anti-NME6 antibody. Overexpression of exogenous FLAG-tagged NME6 led to downregulation of endogenous NME6 at the protein level. c Western blot analysis of recombinant and endogenous NME6 proteins. Human NME6-194-His and NME6-186-His recombinant isoforms were produced in bacteria and purified. The His-tag was partially removed using thrombin and proteins were analyzed by Western blot along with whole cell lysate from MDA-MB-231T (control), using anti-NME6 antibody. Endogenous NME6 and recombinant NME6-186 display similar migration

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