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Fig. 10 | Cell & Bioscience

Fig. 10

From: NME6 is a phosphotransfer-inactive, monomeric NME/NDPK family member and functions in complexes at the interface of mitochondrial inner membrane and matrix

Fig. 10

Co-immunoprecipitation of RCC1L and NME6 reveal physical interaction. MDA-MB-231T cells were either transiently transfected to express a NME6-194-FLAG or b NME6-186-FLAG, or c non-transfected control cells were used. Cell lysates were subjected to pull-down with FLAG-agarose, or anti-NME6 and anti-RCC1L antibodies coupled to protein G-Dynabeads™. Immunoprecipitation with irrelevant immunoglobulins from mouse (IgG-M) or from rabbit (IgG-R) were used as a negative control. Input proteins (I), supernatant depleted of pulled-down proteins (Sn) and eluted proteins (IP) were analyzed by immunoblot with anti-OPA1, anti-RCC1L and anti-NME6 antibodies. Both endogenous and FLAG-NME6 co-immunoprecipitate with RCC1L, but not OPA1. Anti-FLAG does not immunoprecipitate endogenous NME6, confirming that NME6 does not assemble into higher homo-oligomers

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Cell & Bioscience

ISSN: 2045-3701

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