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Fig. 4 | Cell & Bioscience

Fig. 4

From: Single-cell RNA-sequencing reveals distinct immune cell subsets and signaling pathways in IgA nephropathy

Fig. 4

scRNA-seq identified candidate genes and pathways of B cells associated with IgAN. A UMAP illustration of refined B cell clusters annotated by their transcriptomic profiles: B-0 (Red circles), B-1 (Purple circles), B-2 (Blue circles), B-3 (Green circles). B Individual UMAP illustrations showing the refined B cell clusters from CTRL and IgAN. C Heatmap of the marker genes contributed from each B cell subset. D Volcano plot showing the up-regulated DEGs in IgAN B cells compared with that in CTRL. E KEGG pathway (Upper) and GO enrichment (Lower) analysis of the DE genes in CTRL and IgAN B cells. F Statistical analysis of each B cell subset between CTRL and IgAN. CTRL n = 6; IgAN n = 10; Mean ± SEM, *p < 0.05. G Representative violin plots of the marker genes in B cell subsets from CTRL and IgAN. HJ Heatmap of the Spearman correlation of IgAN clinical parameters with the expression levels of DEGs (H), the numbers of each B cell subset (I), and the marker genes of B-2 subset (J). Red and blue boxes indicate positive and negative correlations, respectively. *p < 0.05; **p < 0.01

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