Fig. 2
scRNA-seq discovered the differentially expressed genes (DEGs) in PBMCs from control and IgAN. A Volcano plot of both up- (Red circles) and down-regulated (Blue circles) DEGs in IgAN PBMCs compared with those in CTRL. B, C Violin plots of the up- (B) and down-regulated (C) DE genes in each cell cluster from CTRL (Red violin) and IgAN (Blue violin). D KEGG pathway (Upper) and GO enrichment (Lower) analysis of both up-regulated and down-regulated DEGs. E Flow cytometry validation of HLA-Chigh PBMCs from CTRL (Upper) and IgAN (Lower). F Statistical analysis of HLA-Chigh PBMCs from CTRL and IgAN. CTRL n = 7; IgAN n = 12; Mean ± SEM; *p < 0.01