Skip to main content
Fig. 1 | Cell & Bioscience

Fig. 1

From: Anti-metastatic effect of GV1001 on prostate cancer cells; roles of GnRHR-mediated Gαs-cAMP pathway and AR-YAP1 axis

Fig. 1

Effects of GV1001 on LNCaP-cell migration and tumor metastasis. A Effects of CA, LA, and GV1001 on CRE-reporter activity in HEK293-pcDNA3.1(+) and HEK293-GnRHR cells. The cells were exposed to CA (100 nM), LA (100 nM), or GV1001 (10 μM) for 24 h. Data represent means ± SD (n = 6, ***P < 0.005 vs vehicle-treated control). B Effect of 10 μM GV1001 on CRE-reporter activity in LNCaP cells. Data represent means ± SD (n = 6, ***P < 0.005 vs vehicle-treated control). C mRNA levels of MMP2. RT-qPCR was performed in LNCaP cells treated with LA (100 nM) or GV1001 (10 μM) for 24 h. D Determination of pro-MMP2 and cleaved-MMP2 proteins in LNCaP cells. LNCaP cells were incubated with or without GV1001 (0.1–10 μM) or LA (1–100 nM) for 24 h, and the cell lysates were subjected to immunoblotting using specific antibodies. E Protein expressions of EMT-related genes in LNCaP cells. LNCaP cells were incubated with GV1001 (0.1–10 μM) for 24 h. F Effects of GV1001 or LA on the spheroid formation of LNCaP cells. LNCaP cells were seeded in a 96-well ULA plate and treated with LA (100 nM) or GV1001 (10 μM) for 96 h. The number of spheroids (average diameter > 100 μm) was determined. Scale bar = 300 μm. Data represent means ± SD (n = 4, *P < 0.05 vs vehicle-treated control). GI Spleen-liver metastasis in BALB/c-nude mice implanted with LNCaP cells into their spleens. After implantation, the mice were divided into 3 groups (n = 7–10) and then injected with vehicle (sterile water 100 μl, daily, sc), GV1001 (10 mg/kg, daily, sc) or LA (0.1 mg/kg, daily, sc) for 4 weeks. G Representative pictures of the metastatic liver tumors from each group are shown. Scale bar = 10 mm. H Statistical analysis of the incidence of liver metastatic colonization (the percentage of mice with metastatic tumor burden in the liver) and weights of tumor-bearing livers. Data represent means ± SD (n = 7–10, P = 0.044 vs vehicle-treated control, The P-value was calculated by two-sided Fisher exact test.). I Histological analysis of the metastasized-liver section. Data represent means ± SD (n = 7–10, **P < 0.01 vs vehicle-treated control)

Back to article page