Fig. 2

METH inhibits viral restriction factors. A–E Monocytes from human peripheral blood were treated with METH (150 μM) for the indicated times or at the indicated concentrations for 24 h. A The cellular RNAs were extracted and subjected to the real-time PCR for IFN-α, IFN-β, and IFN-λ1 mRNA expression, the culture supernatants were collected and subjected to ELISA for IFN-λ1 protein expression. B–E The cellular RNAs or proteins were extracted and subjected to the real-time PCR or Western blot assays. F, G Monocytes were treated with METH (150 μM) for the indicated times. Cellular miRNAs were quantified by the real-time PCR. RNU48 was used as a control gene. miRNAs in the culture supernatants were quantified by the real-time PCR. Synthetic Caenorhabditis elegans miRNA-39 (cel-miR-39) was used as a spiked-in control miRNA for normalization. Data shown were the mean ± SD of three independent experiments with monocytes from three different donors (*P < 0.05, **P < 0.01)