Fig. 4

PRCC inhibited HCC cell metastasis in vitro and in vivo. a Cell wound and healing assay and the quantitative analysis results (*, P < 0.05; **, P < 0.01; ***, P < 0.001). b Transwell assay and the quantitative analysis results (*, P < 0.05; **, P < 0.01; ***, P < 0.001). c Expression of the several metastasis-related genes examined by RT-qPCR (**, P < 0.01). d Expression of the metastasis-related proteins examined by Western blot. The numbers below the bands were the ratios of the gray values of each indicator to that of GAPDH, respectively. e Metastatic tumor nodules in lungs of three groups of nude mice. f The numbers of metastatic tumor nodules in lungs of three groups (***, P < 0.001). g The metastatic tumor nodules in lungs, livers and kidneys of nude mice by HE staining. Bar = 100 μm