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Fig. 2 | Cell & Bioscience

Fig. 2

From: PRCC reduces the sensitivity of cancer cells to DNA damage by inhibiting JNK and ATM/ATR pathways and results in a poor prognosis in hepatocellular carcinoma

Fig. 2

PRCC had no significant effect on the proliferation of HCC cells. a PRCC was detected by RT-qPCR in different HCC cell lines (**, P < 0.01; ***, P < 0.001). b PRCC was detected by Western blot in HCC cell lines. The numbers below the bands were the ratios of the gray values of PRCC normalized to that of GAPDH. c Fluorescence microscopy showed that PRCC located in nuclei. Bar = 25 μm. d PRCC was detected by Western blot in the PRCC OE and KD cell sublines. The numbers below the bands were the ratios of the gray values of PRCC to that of GAPDH. e CCK8 assay showed that PRCC had no significant effect on the proliferation of HCC cells. f Colony formation assay showed that PRCC inhibited the colony forming ability of HCC cells. (*, P < 0.05; **, P < 0.01). All data were representative of three independent experiments

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